5L4Z

Crystal structure of enzyme in purine metabolism

Summary for 5L4Z

• Entry DOI: 10.2210/pdb5l4z/pdb
• Descriptor: Cytosolic purine 5'-nucleotidase, GLYCEROL (3 entities in total)
• Functional Keywords: enzyme, purine, hydrolase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: P49902
• Total number of polymer chains: 1
• Total formula weight: 65315.17

Authors

Hnizda, A.,Pachl, P.,Rezacova, P. (deposition date: 2016-05-27, release date: 2016-09-21, Last modification date: 2024-01-10)

Primary citation

Hnizda, A.,Skerlova, J.,Fabry, M.,Pachl, P.,Sinalova, M.,Vrzal, L.,Man, P.,Novak, P.,Rezacova, P.,Veverka, V.
Oligomeric interface modulation causes misregulation of purine 5 -nucleotidase in relapsed leukemia.
Bmc Biol., 14:91-91, 2016

PubMed Abstract: Relapsed acute lymphoblastic leukemia (ALL) is one of the main causes of mortality in childhood malignancies. Previous genetic studies demonstrated that chemoresistant ALL is driven by activating mutations in NT5C2, the gene encoding cytosolic 5´-nucleotidase (cN-II). However, molecular mechanisms underlying this hyperactivation are still unknown. Here, we present kinetic and structural properties of cN-II variants that represent 75 % of mutated alleles in patients who experience relapsed ALL (R367Q, R238W and L375F).

PubMed: 27756303
DOI: 10.1186/s12915-016-0313-y

Experimental method

X-RAY DIFFRACTION (1.84 Å)