5L4Z

Crystal structure of enzyme in purine metabolism

5L4Z の概要

• エントリーDOI: 10.2210/pdb5l4z/pdb
• 分子名称: Cytosolic purine 5'-nucleotidase, GLYCEROL (3 entities in total)
• 機能のキーワード: enzyme, purine, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm: P49902
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65315.17

構造登録者

Hnizda, A.,Pachl, P.,Rezacova, P. (登録日: 2016-05-27, 公開日: 2016-09-21, 最終更新日: 2024-01-10)

主引用文献

Hnizda, A.,Skerlova, J.,Fabry, M.,Pachl, P.,Sinalova, M.,Vrzal, L.,Man, P.,Novak, P.,Rezacova, P.,Veverka, V.
Oligomeric interface modulation causes misregulation of purine 5 -nucleotidase in relapsed leukemia.
Bmc Biol., 14:91-91, 2016

PubMed Abstract: Relapsed acute lymphoblastic leukemia (ALL) is one of the main causes of mortality in childhood malignancies. Previous genetic studies demonstrated that chemoresistant ALL is driven by activating mutations in NT5C2, the gene encoding cytosolic 5´-nucleotidase (cN-II). However, molecular mechanisms underlying this hyperactivation are still unknown. Here, we present kinetic and structural properties of cN-II variants that represent 75 % of mutated alleles in patients who experience relapsed ALL (R367Q, R238W and L375F).

PubMed: 27756303
DOI: 10.1186/s12915-016-0313-y

実験手法

X-RAY DIFFRACTION (1.84 Å)