5KTM

Crystal structure of Pyrococcus horikoshii quinolinate synthase (NadA) with a bound Fe4S4 cluster

5KTM の概要

• エントリーDOI: 10.2210/pdb5ktm/pdb
• 関連するPDBエントリー: 5KTN 5KTO 5KTP 5KTR 5KTS 5KTT
• 分子名称: Quinolinate synthase A, IRON/SULFUR CLUSTER, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: dehydratase, iron-sulfur cluster, holoenzyme, biosynthetic enzyme, transferase
• 由来する生物種: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34969.53

構造登録者

Fenwick, M.K.,Ealick, S.E. (登録日: 2016-07-12, 公開日: 2016-07-27, 最終更新日: 2023-10-04)

主引用文献

Fenwick, M.K.,Ealick, S.E.
Crystal Structures of the Iron-Sulfur Cluster-Dependent Quinolinate Synthase in Complex with Dihydroxyacetone Phosphate, Iminoaspartate Analogues, and Quinolinate.
Biochemistry, 55:4135-4139, 2016

PubMed Abstract: The quinolinate synthase of prokaryotes and photosynthetic eukaryotes, NadA, contains a [4Fe-4S] cluster with unknown function. We report crystal structures of Pyrococcus horikoshii NadA in complex with dihydroxyacetone phosphate (DHAP), iminoaspartate analogues, and quinolinate. DHAP adopts a nearly planar conformation and chelates the [4Fe-4S] cluster via its keto and hydroxyl groups. The active site architecture suggests that the cluster acts as a Lewis acid in enediolate formation, like zinc in class II aldolases. The DHAP and putative iminoaspartate structures suggest a model for a condensed intermediate. The ensemble of structures suggests a two-state system, which may be exploited in early steps.

PubMed: 27404889
DOI: 10.1021/acs.biochem.6b00626

実験手法

X-RAY DIFFRACTION (1.44 Å)