5KKV

Metal-mediated coiled-coil GCN4-p2L peptide assembly crystal

5KKV の概要

• エントリーDOI: 10.2210/pdb5kkv/pdb
• 分子名称: GCN4-p2L (2 entities in total)
• 機能のキーワード: gcn4 coiled-coil, protein fibril
• 由来する生物種: Eukaryota
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4295.93

構造登録者

Nepal, M.,Sheedlo, M.J.,Das, C.,Chmielewski, J. (登録日: 2016-06-22, 公開日: 2016-08-24, 最終更新日: 2024-10-16)

主引用文献

Nepal, M.,Sheedlo, M.J.,Das, C.,Chmielewski, J.
Accessing Three-Dimensional Crystals with Incorporated Guests through Metal-Directed Coiled-Coil Peptide Assembly.
J.Am.Chem.Soc., 138:11051-11057, 2016

PubMed Abstract: Obtaining three-dimensional (3D) protein and peptide crystals on demand requires a precisely orchestrated hierarchical assembly of biopolymer building blocks. In this work, we disclose a metal-ion-mediated strategy to assemble trimeric coiled-coil peptides in a head-to-tail fashion into linear strands with interstrand interactions. This design led to hexagonal 3D peptide crystal formation within 30 min in the presence of divalent metal ions. The crystal morphology could be controlled by varying the metal ion/peptide ratio, resulting in hexagonal discs to rods. Diffraction studies elucidated the head-to-tail arrangement of the coiled-coil linear strands and their hexagonal, antiparallel packing within the crystal. Unsatisfied ligands at the hexagonal ends of the crystals were harnessed as a powerful means to direct His-tagged fluorophores to distinct locations within the crystals. Overall, the designed hierarchical assembly provides a facile means to obtain 3D peptide crystals and incorporate His-tag-based cargoes and may have potential use in drug delivery and sensor design.

PubMed: 27500907
DOI: 10.1021/jacs.6b06708

実験手法

X-RAY DIFFRACTION (1.9 Å)