5KHR

Model of human Anaphase-promoting complex/Cyclosome complex (APC15 deletion mutant) in complex with the E2 UBE2C/UBCH10 poised for ubiquitin ligation to substrate (APC/C-CDC20-substrate-UBE2C)

Summary for 5KHR

• Entry DOI: 10.2210/pdb5khr/pdb
• Related: 5KHU
• EMDB information: 4021 4025
• Descriptor: Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 13, Anaphase-promoting complex subunit 2, ... (16 entities in total)
• Functional Keywords: ubiquitination, cell cycle, protein complex, mitosis
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q9BS18 P30260 Q8NHZ8; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome : Q12834 Q13042; Cytoplasm : Q9NYG5 Q96DE5
• Total number of polymer chains: 21
• Total formula weight: 1231156.22

Authors

VanderLinden, R.,Yamaguchi, M.,Dube, P.,Haselbach, D.,Stark, H.,Schulman, B.A. (deposition date: 2016-06-15, release date: 2016-08-24, Last modification date: 2024-03-06)

• Primary citation: Yamaguchi, M.,VanderLinden, R.,Weissmann, F.,Qiao, R.,Dube, P.,Brown, N.G.,Haselbach, D.,Zhang, W.,Sidhu, S.S.,Peters, J.M.,Stark, H.,Schulman, B.A.; Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.; Mol.Cell, 63:593-607, 2016; PubMed: 27522463; DOI: 10.1016/j.molcel.2016.07.003

Experimental method

ELECTRON MICROSCOPY (6.1 Å)