5KHF

Fitted structure of rubella virus capsid protein

Summary for 5KHF

• Entry DOI: 10.2210/pdb5khf/pdb
• Related: 5KHC 5KHE
• EMDB information: 8248 8249 8250 8251
• Descriptor: capsid protein (2 entities in total)
• Functional Keywords: rubella virus capsid protein, viral protein
• Biological source: Rubella virus (RUBV)
• Cellular location: Capsid protein: Virion . E1 envelope glycoprotein: Virion membrane ; Single-pass type I membrane protein . E2 envelope glycoprotein: Virion membrane ; Single-pass type I membrane protein : P07566
• Total number of polymer chains: 2
• Total formula weight: 60032.66

Authors

Mangala Prasad, V.,Klose, T.,Rossmann, M.G. (deposition date: 2016-06-14, release date: 2017-05-10, Last modification date: 2024-10-23)

Primary citation

Mangala Prasad, V.,Klose, T.,Rossmann, M.G.
Assembly, maturation and three-dimensional helical structure of the teratogenic rubella virus.
PLoS Pathog., 13:e1006377-e1006377, 2017

PubMed Abstract: Viral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe fetal defects. However, structural information about the rubella virus has been lacking due to the pleomorphic nature of the virions. Here we report a helical structure of rubella virions using cryo-electron tomography. Sub-tomogram averaging of the surface spikes established the relative positions of the viral glycoproteins, which differed from the earlier icosahedral models of the virus. Tomographic analyses of in vitro assembled nucleocapsids and virions provide a template for viral assembly. Comparisons of immature and mature virions show large rearrangements in the glycoproteins that may be essential for forming the infectious virions. These results present the first known example of a helical membrane-enveloped virus, while also providing a structural basis for its assembly and maturation pathway.

PubMed: 28575072
DOI: 10.1371/journal.ppat.1006377

Experimental method

ELECTRON MICROSCOPY (35 Å)