5KHC

Structure of rubella virus E1 glycoprotein ectodomain fitted into sub-tomogram averaged surface spike density of rubella virus

5KHC の概要

• エントリーDOI: 10.2210/pdb5khc/pdb
• 関連するPDBエントリー: 5KHE 5KHF
• EMDBエントリー: 8248 8249 8250 8251
• 分子名称: E1 glycoprotein (1 entity in total)
• 機能のキーワード: rubella virus, surface glycoprotein spike, e1-e2 heterodimer, viral protein
• 由来する生物種: Rubella virus (RUBV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50573.35

構造登録者

Mangala Prasad, V.,Klose, T.,Rossmann, M.G. (登録日: 2016-06-14, 公開日: 2017-05-10, 最終更新日: 2024-10-30)

主引用文献

Mangala Prasad, V.,Klose, T.,Rossmann, M.G.
Assembly, maturation and three-dimensional helical structure of the teratogenic rubella virus.
PLoS Pathog., 13:e1006377-e1006377, 2017

PubMed Abstract: Viral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe fetal defects. However, structural information about the rubella virus has been lacking due to the pleomorphic nature of the virions. Here we report a helical structure of rubella virions using cryo-electron tomography. Sub-tomogram averaging of the surface spikes established the relative positions of the viral glycoproteins, which differed from the earlier icosahedral models of the virus. Tomographic analyses of in vitro assembled nucleocapsids and virions provide a template for viral assembly. Comparisons of immature and mature virions show large rearrangements in the glycoproteins that may be essential for forming the infectious virions. These results present the first known example of a helical membrane-enveloped virus, while also providing a structural basis for its assembly and maturation pathway.

PubMed: 28575072
DOI: 10.1371/journal.ppat.1006377

実験手法

ELECTRON MICROSCOPY (11.1 Å)