5KFM

Human DNA polymerase eta-DNA ternary complex with Sp-dATP-alpha-S: ground state at pH7.0 (K+ MES) with 1 Ca2+ ion

5KFM の概要

• エントリーDOI: 10.2210/pdb5kfm/pdb
• 分子名称: DNA polymerase eta, DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*T)-3'), ... (7 entities in total)
• 機能のキーワード: in crystallo reaction, dna polymerase, metal ion dependent catalysis, replication, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 55505.32

構造登録者

Gao, Y.,Yang, W. (登録日: 2016-06-12, 公開日: 2016-06-22, 最終更新日: 2023-09-27)

主引用文献

Gao, Y.,Yang, W.
Capture of a third Mg2+ is essential for catalyzing DNA synthesis.
Science, 352:1334-1337, 2016

PubMed Abstract: It is generally assumed that an enzyme-substrate (ES) complex contains all components necessary for catalysis and that conversion to products occurs by rearrangement of atoms, protons, and electrons. However, we find that DNA synthesis does not occur in a fully assembled DNA polymerase-DNA-deoxynucleoside triphosphate complex with two canonical metal ions bound. Using time-resolved x-ray crystallography, we show that the phosphoryltransfer reaction takes place only after the ES complex captures a third divalent cation that is not coordinated by the enzyme. Binding of the third cation is incompatible with the basal ES complex and requires thermal activation of the ES for entry. It is likely that the third cation provides the ultimate boost over the energy barrier to catalysis of DNA synthesis.

PubMed: 27284197
DOI: 10.1126/science.aad9633

実験手法

X-RAY DIFFRACTION (1.6 Å)