5KEK

Structure Determination of a Self-Assembling DNA Crystal

5KEK の概要

• エントリーDOI: 10.2210/pdb5kek/pdb
• 関連するPDBエントリー: 5KEO
• 分子名称: DNA (5'-D(*GP*AP*GP*CP*AP*GP*AP*CP*CP*TP*GP*AP*CP*GP*AP*CP*AP*CP*TP*CP*A)-3'), DNA (5'-D(P*CP*GP*TP*CP*A)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*GP*TP*GP*T)-3'), ... (5 entities in total)
• 機能のキーワード: structural dna nanoechnology, self-assembled crystals, self-assembly, dna
• 由来する生物種: Endothia gyrosa; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 13071.40

構造登録者

Simmons, C.R.,Birktoft, J.J.,Seeman, N.C.,Yan, H. (登録日: 2016-06-09, 公開日: 2016-08-10, 最終更新日: 2024-03-06)

主引用文献

Simmons, C.R.,Zhang, F.,Birktoft, J.J.,Qi, X.,Han, D.,Liu, Y.,Sha, R.,Abdallah, H.O.,Hernandez, C.,Ohayon, Y.P.,Seeman, N.C.,Yan, H.
Construction and Structure Determination of a Three-Dimensional DNA Crystal.
J.Am.Chem.Soc., 138:10047-10054, 2016

PubMed Abstract: Structural DNA nanotechnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals of structural DNA nanotechnology is to construct three-dimensional (3D) crystalline lattices. Here we present a new DNA motif and a strategy that has led to the assembly of a 3D lattice. We have determined the X-ray crystal structures of two related constructs to 3.1 Å resolution using bromine-derivatized crystals. The motif we used employs a five-nucleotide repeating sequence that weaves through a series of two-turn DNA duplexes. The duplexes are tied into a layered structure that is organized and dictated by a concert of four-arm junctions; these in turn assemble into continuous arrays facilitated by sequence-specific sticky-ended cohesion. The 3D X-ray structure of these DNA crystals holds promise for the design of new structural motifs to create programmable 3D DNA lattices with atomic spatial resolution. The two arrays differ by the use of four or six repeats of the five-nucleotide units in the repeating but statistically disordered central strand. In addition, we report a 2D rhombuslike array formed from similar components.

PubMed: 27447429
DOI: 10.1021/jacs.6b06508

実験手法

X-RAY DIFFRACTION (3.098 Å)