5KAZ

Human SH2D1B structure

5KAZ の概要

• エントリーDOI: 10.2210/pdb5kaz/pdb
• 分子名称: SH2 domain-containing protein 1B, SULFATE ION (3 entities in total)
• 機能のキーワード: sh2d1b, eat2, slam, sap, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12194.21

構造登録者

Taha, M.,Nezerwa, E.,Nam, H.-J. (登録日: 2016-06-02, 公開日: 2017-04-12, 最終更新日: 2024-02-28)

主引用文献

Taha, M.,Nezerwa, E.,Nam, H.J.
The X-ray Crystallographic Structure of Human EAT2 (SH2D1B).
Protein Pept. Lett., 23:862-866, 2016

PubMed Abstract: Ewing's Sarcoma transcript-2 (EAT2) also known as SH2D1B is involved in regulation of signalling lymphocytic activation molecule (SLAM) family receptor functions. Cytoplasmic tails of SLAM family receptors contain tyrosine residues which mediate the downstream signal transduction through their phosphorylation. EAT2, composed of a single SH2 domain and a short C-terminal tail, binds to the phosphotyrosine residues and regulates SLAM family receptor signalling. We have determined the crystal structure of the human EAT2 protein in an unliganded form. Compared with the mouse EAT2-peptide complex structure, we observe conformational differences in the loops involved in ligand binding. When compared with SAP, the other single SH2 domain protein in human, EAT2 shows similar binding energies to unphosphorylated ligands. This is inconsistent to the previous data showing low affinity of EAT2 toward unphosphorylated peptides compared to SAP which shows high affinity. Additional factors other than the SH2 domains may contribute to the reported differences.

PubMed: 27586300

実験手法

X-RAY DIFFRACTION (1.7 Å)