5K9M
Crystal Structure of PriB Binary Complex with Product Diphosphate
Summary for 5K9M
| Entry DOI | 10.2210/pdb5k9m/pdb |
| Related | 5INJ 5JXM |
| Descriptor | PriB Prenyltransferase, PYROPHOSPHATE 2-, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total) |
| Functional Keywords | prenyltransferase, diphosphate, indole-pt, abba family, pt fold, structural genomics, psi-2, protein structure initiative, enzyme discovery for natural product biosynthesis, natpro, transferase |
| Biological source | Streptomyces sp. RM-5-8 |
| Total number of polymer chains | 1 |
| Total formula weight | 43698.80 |
| Authors | Cao, H.,Elshahawi, S.,Benach, J.,Wasserman, S.R.,Morisco, L.L.,Koss, J.W.,Thorson, J.S.,Phillips Jr., G.N.,Enzyme Discovery for Natural Product Biosynthesis (NatPro) (deposition date: 2016-06-01, release date: 2016-06-15, Last modification date: 2023-09-27) |
| Primary citation | Elshahawi, S.I.,Cao, H.,Shaaban, K.A.,Ponomareva, L.V.,Subramanian, T.,Farman, M.L.,Spielmann, H.P.,Phillips, G.N.,Thorson, J.S.,Singh, S. Structure and specificity of a permissive bacterial C-prenyltransferase. Nat. Chem. Biol., 13:366-368, 2017 Cited by PubMed Abstract: This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug. PubMed: 28166207DOI: 10.1038/nchembio.2285 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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