5K5R

AspA-32mer DNA,crystal form 2

「4RU7」から置き換えられました

5K5R の概要

• エントリーDOI: 10.2210/pdb5k5r/pdb
• 分子名称: AspA, DNA (32-MER), PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: aspa, centromere, dna, partition, archaea, transcription-dna complex, transcription/dna
• 由来する生物種: Sulfolobus sp. NOB8H2; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 89082.96

構造登録者

Schumacher, M. (登録日: 2016-05-23, 公開日: 2016-06-15, 最終更新日: 2023-09-27)

主引用文献

Schumacher, M.A.,Tonthat, N.K.,Lee, J.,Rodriguez-Castaneda, F.A.,Chinnam, N.B.,Kalliomaa-Sanford, A.K.,Ng, I.W.,Barge, M.T.,Shaw, P.L.,Barilla, D.
Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
Science, 349:1120-1124, 2015

PubMed Abstract: Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

PubMed: 26339031
DOI: 10.1126/science.aaa9046

実験手法

X-RAY DIFFRACTION (3.09 Å)