5K35

Structure of the Legionella effector, AnkB, in complex with human Skp1

5K35 の概要

• エントリーDOI: 10.2210/pdb5k35/pdb
• 関連するPDBエントリー: 5K34
• 分子名称: Ankyrin-repeat protein B, S-phase kinase-associated protein 1 (3 entities in total)
• 機能のキーワード: bacterial effector, host-pathogen interaction, f-box protein, ankyrin repeats, structural genomics, montreal-kingston bacterial structural genomics initiative, bsgi, protein binding
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39908.28

構造登録者

Wong, K.,Kozlov, G.,Gehring, K.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2016-05-19, 公開日: 2017-01-25, 最終更新日: 2024-03-06)

主引用文献

Wong, K.,Perpich, J.D.,Kozlov, G.,Cygler, M.,Abu Kwaik, Y.,Gehring, K.
Structural Mimicry by a Bacterial F Box Effector Hijacks the Host Ubiquitin-Proteasome System.
Structure, 25:376-383, 2017

PubMed Abstract: Ankyrin B (AnkB/LegAU13) is a translocated F box effector essential for the intracellular replication of the pathogen Legionella pneumophila. AnkB co-opts a host ubiquitin ligase to decorate the pathogen-containing vacuole with K-linked polyubiquitinated proteins and degrade host proteins as a source of energy. Here, we report that AnkB commandeers the host ubiquitin-proteasome system through mimicry of two eukaryotic protein domains. Using X-ray crystallography, we determined the 3D structure of AnkB in complex with Skp1, a component of the human SCF ubiquitination ligase. The structure confirms that AnkB contains an N-terminal F box similar to Skp2 and a C-terminal substrate-binding domain similar to eukaryotic ankyrin repeats. We identified crucial amino acids in the substrate-binding domain of AnkB and showed them to be essential for the function of AnkB in L. pneumophila intracellular proliferation. The study reveals how Legionella uses molecular mimicry to manipulate the host ubiquitination pathway and proliferate intracellularly.

PubMed: 28111017
DOI: 10.1016/j.str.2016.12.015

実験手法

X-RAY DIFFRACTION (2.85 Å)