5JVU

The neck-linker and alpha 7 helix of Drosophila melanogaster kinesin-1 fused to EB1

5JVU の概要

• エントリーDOI: 10.2210/pdb5jvu/pdb
• 関連するPDBエントリー: 5JV3 5JVM 5JVP 5JVR 5JVS
• 分子名称: Chimera protein of Kinesin heavy chain and Microtubule-associated protein RP/EB family member 1, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: kinesin, coiled-coil, motor protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20208.67

構造登録者

Phillips, R.K.,Rayment, I. (登録日: 2016-05-11, 公開日: 2016-08-03, 最終更新日: 2023-09-27)

主引用文献

Phillips, R.K.,Peter, L.G.,Gilbert, S.P.,Rayment, I.
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
J.Biol.Chem., 291:20372-20386, 2016

PubMed Abstract: Kinesin-1, -2, -5, and -7 generate processive hand-over-hand 8-nm steps to transport intracellular cargoes toward the microtubule plus end. This processive motility requires gating mechanisms to coordinate the mechanochemical cycles of the two motor heads to sustain the processive run. A key structural element believed to regulate the degree of processivity is the neck-linker, a short peptide of 12-18 residues, which connects the motor domain to its coiled-coil stalk. Although a shorter neck-linker has been correlated with longer run lengths, the structural data to support this hypothesis have been lacking. To test this hypothesis, seven kinesin structures were determined by x-ray crystallography. Each included the neck-linker motif, followed by helix α7 that constitutes the start of the coiled-coil stalk. In the majority of the structures, the neck-linker length differed from predictions because helix α7, which initiates the coiled-coil, started earlier in the sequence than predicted. A further examination of structures in the Protein Data Bank reveals that there is a great disparity between the predicted and observed starting residues. This suggests that an accurate prediction of the start of a coiled-coil is currently difficult to achieve. These results are significant because they now exclude simple comparisons between members of the kinesin superfamily and add a further layer of complexity when interpreting the results of mutagenesis or protein fusion. They also re-emphasize the need to consider factors beyond the kinesin neck-linker motif when attempting to understand how inter-head communication is tuned to achieve the degree of processivity required for cellular function.

PubMed: 27462072
DOI: 10.1074/jbc.M116.737577

実験手法

X-RAY DIFFRACTION (1.948 Å)