5JV3

The neck-linker and alpha 7 helix of Homo sapiens Eg5 fused to EB1

Summary for 5JV3

• Entry DOI: 10.2210/pdb5jv3/pdb
• Related: 5JVM 5JVP 5JVR 5JVS 5JVU 5JX1
• Descriptor: Chimera protein of Kinesin-like protein KIF11 and Microtubule-associated protein RP/EB family member 1, CALCIUM ION (3 entities in total)
• Functional Keywords: kinesin, coiled-coil, motor protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 35860.73

Authors

Phillips, R.K.,Rayment, I. (deposition date: 2016-05-10, release date: 2016-08-03, Last modification date: 2023-09-27)

Primary citation

Phillips, R.K.,Peter, L.G.,Gilbert, S.P.,Rayment, I.
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
J.Biol.Chem., 291:20372-20386, 2016

PubMed Abstract: Kinesin-1, -2, -5, and -7 generate processive hand-over-hand 8-nm steps to transport intracellular cargoes toward the microtubule plus end. This processive motility requires gating mechanisms to coordinate the mechanochemical cycles of the two motor heads to sustain the processive run. A key structural element believed to regulate the degree of processivity is the neck-linker, a short peptide of 12-18 residues, which connects the motor domain to its coiled-coil stalk. Although a shorter neck-linker has been correlated with longer run lengths, the structural data to support this hypothesis have been lacking. To test this hypothesis, seven kinesin structures were determined by x-ray crystallography. Each included the neck-linker motif, followed by helix α7 that constitutes the start of the coiled-coil stalk. In the majority of the structures, the neck-linker length differed from predictions because helix α7, which initiates the coiled-coil, started earlier in the sequence than predicted. A further examination of structures in the Protein Data Bank reveals that there is a great disparity between the predicted and observed starting residues. This suggests that an accurate prediction of the start of a coiled-coil is currently difficult to achieve. These results are significant because they now exclude simple comparisons between members of the kinesin superfamily and add a further layer of complexity when interpreting the results of mutagenesis or protein fusion. They also re-emphasize the need to consider factors beyond the kinesin neck-linker motif when attempting to understand how inter-head communication is tuned to achieve the degree of processivity required for cellular function.

PubMed: 27462072
DOI: 10.1074/jbc.M116.737577

Experimental method

X-RAY DIFFRACTION (2.006 Å)