5JSI

Structure of membrane protein

5JSI の概要

• エントリーDOI: 10.2210/pdb5jsi/pdb
• 分子名称: Bacteriorhodopsin, EICOSANE, IODIDE ION, ... (6 entities in total)
• 機能のキーワード: membrane protein, iodide, unknown function, transferase
• 由来する生物種: Candidatus Actinomarina minuta; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58487.49

構造登録者

Melnikov, I.,Polovinkin, V.,Kovalev, K.,Shevchenko, V.,Gushchin, I.,Popov, A.,Gordeliy, V. (登録日: 2016-05-08, 公開日: 2017-05-31, 最終更新日: 2025-04-09)

主引用文献

Melnikov, I.,Polovinkin, V.,Kovalev, K.,Gushchin, I.,Shevtsov, M.,Shevchenko, V.,Mishin, A.,Alekseev, A.,Rodriguez-Valera, F.,Borshchevskiy, V.,Cherezov, V.,Leonard, G.A.,Gordeliy, V.,Popov, A.
Fast iodide-SAD phasing for high-throughput membrane protein structure determination.
Sci Adv, 3:e1602952-e1602952, 2017

PubMed Abstract: We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide-single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins-the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein-coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.

PubMed: 28508075
DOI: 10.1126/sciadv.1602952

実験手法

X-RAY DIFFRACTION (2 Å)