5JRF

Crystal structure of the light-driven sodium pump KR2 bound with iodide ions

Summary for 5JRF

• Entry DOI: 10.2210/pdb5jrf/pdb
• Related: 4XTL
• Descriptor: Sodium pumping rhodopsin, EICOSANE, IODIDE ION, ... (4 entities in total)
• Functional Keywords: membrane protein, sodium pump, iodide, transport protein
• Biological source: Dokdonia eikasta
• Total number of polymer chains: 1
• Total formula weight: 37677.46

Authors

Melnikov, I.,Polovinkin, V.,Kovalev, K.,Shevchenko, V.,Gushchin, I.,Popov, A.,Gordeliy, V. (deposition date: 2016-05-06, release date: 2017-05-31)

Primary citation

Melnikov, I.,Polovinkin, V.,Kovalev, K.,Gushchin, I.,Shevtsov, M.,Shevchenko, V.,Mishin, A.,Alekseev, A.,Rodriguez-Valera, F.,Borshchevskiy, V.,Cherezov, V.,Leonard, G.A.,Gordeliy, V.,Popov, A.
Fast iodide-SAD phasing for high-throughput membrane protein structure determination.
Sci Adv, 3:e1602952-e1602952, 2017

PubMed Abstract: We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide-single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins-the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein-coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.

PubMed: 28508075
DOI: 10.1126/sciadv.1602952

Experimental method

X-RAY DIFFRACTION (2.5 Å)