5JMX

Crystal Structure of BcII metallo-beta-lactamase in complex with DZ-305

5JMX の概要

• エントリーDOI: 10.2210/pdb5jmx/pdb
• 分子名称: Metallo-beta-lactamase type 2, ZINC ION, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: antimicrobial resistance, metallo beta lactamase, inhibitor, hydrolase
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25696.91

構造登録者

Stepanovs, D.,McDonough, M.A.,Schofield, C.J.,Zhang, D.,Brem, J. (登録日: 2016-04-29, 公開日: 2017-05-24, 最終更新日: 2024-01-10)

主引用文献

Zhang, D.,Markoulides, M.S.,Stepanovs, D.,Rydzik, A.M.,El-Hussein, A.,Bon, C.,Kamps, J.J.A.G.,Umland, K.D.,Collins, P.M.,Cahill, S.T.,Wang, D.Y.,von Delft, F.,Brem, J.,McDonough, M.A.,Schofield, C.J.
Structure activity relationship studies on rhodanines and derived enethiol inhibitors of metallo-beta-lactamases.
Bioorg. Med. Chem., 26:2928-2936, 2018

PubMed Abstract: Metallo-β-lactamases (MBLs) enable bacterial resistance to almost all classes of β-lactam antibiotics. We report studies on enethiol containing MBL inhibitors, which were prepared by rhodanine hydrolysis. The enethiols inhibit MBLs from different subclasses. Crystallographic analyses reveal that the enethiol sulphur displaces the di-Zn(II) ion bridging 'hydrolytic' water. In some, but not all, cases biophysical analyses provide evidence that rhodanine/enethiol inhibition involves formation of a ternary MBL enethiol rhodanine complex. The results demonstrate how low molecular weight active site Zn(II) chelating compounds can inhibit a range of clinically relevant MBLs and provide additional evidence for the potential of rhodanines to be hydrolysed to potent inhibitors of MBL protein fold and, maybe, other metallo-enzymes, perhaps contributing to the complex biological effects of rhodanines. The results imply that any medicinal chemistry studies employing rhodanines (and related scaffolds) as inhibitors should as a matter of course include testing of their hydrolysis products.

PubMed: 29655609
DOI: 10.1016/j.bmc.2018.02.043

実験手法

X-RAY DIFFRACTION (1.44 Å)