5JIG

Crytsal structure of Wss1 from S. pombe

5JIG の概要

• エントリーDOI: 10.2210/pdb5jig/pdb
• 分子名称: Ubiquitin and WLM domain-containing metalloprotease SPCC1442.07c, NICKEL (II) ION, OXYGEN MOLECULE, ... (4 entities in total)
• 機能のキーワード: metalloprotease, dna-repair, endoprotease, regulation, hydrolase
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
• 細胞内の位置: Cytoplasm : O94580
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15086.45

構造登録者

Groll, M.,Stingele, J.,Boulton, S. (登録日: 2016-04-22, 公開日: 2016-11-09, 最終更新日: 2024-05-08)

主引用文献

Stingele, J.,Bellelli, R.,Alte, F.,Hewitt, G.,Sarek, G.,Maslen, S.L.,Tsutakawa, S.E.,Borg, A.,Kjr, S.,Tainer, J.A.,Skehel, J.M.,Groll, M.,Boulton, S.J.
Mechanism and Regulation of DNA-Protein Crosslink Repair by the DNA-Dependent Metalloprotease SPRTN.
Mol.Cell, 64:688-703, 2016

PubMed Abstract: Covalent DNA-protein crosslinks (DPCs) are toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription. Little was known about DPC-specific repair mechanisms until the recent identification of a DPC-processing protease in yeast. The existence of a DPC protease in higher eukaryotes is inferred from data in Xenopus laevis egg extracts, but its identity remains elusive. Here we identify the metalloprotease SPRTN as the DPC protease acting in metazoans. Loss of SPRTN results in failure to repair DPCs and hypersensitivity to DPC-inducing agents. SPRTN accomplishes DPC processing through a unique DNA-induced protease activity, which is controlled by several sophisticated regulatory mechanisms. Cellular, biochemical, and structural studies define a DNA switch triggering its protease activity, a ubiquitin switch controlling SPRTN chromatin accessibility, and regulatory autocatalytic cleavage. Our data also provide a molecular explanation on how SPRTN deficiency causes the premature aging and cancer predisposition disorder Ruijs-Aalfs syndrome.

PubMed: 27871365
DOI: 10.1016/j.molcel.2016.09.031

実験手法

X-RAY DIFFRACTION (1 Å)