5JHL

Crystal structure of zika virus envelope protein in complex with a flavivirus broadly-protective antibody

Summary for 5JHL

• Entry DOI: 10.2210/pdb5jhl/pdb
• Related: 5JHM
• Descriptor: envelope protein, Antibody Heavy chain, antibody Light chain (3 entities in total)
• Functional Keywords: zika virus, envelope protein, flavivirus, broadly-protective antibody, viral protein-immune system complex, viral protein/immune system
• Biological source: Zika virus (ZIKV); More
• Total number of polymer chains: 3
• Total formula weight: 94391.60

Authors

Dai, L.,Shi, Y.,Qi, J.,Gao, G.F. (deposition date: 2016-04-21, release date: 2016-05-11, Last modification date: 2024-11-06)

Primary citation

Dai, L.,Song, J.,Lu, X.,Deng, Y.Q.,Musyoki, A.M.,Cheng, H.,Zhang, Y.,Yuan, Y.,Song, H.,Haywood, J.,Xiao, H.,Yan, J.,Shi, Y.,Qin, C.F.,Qi, J.,Gao, G.F.
Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody.
Cell Host Microbe, 19:696-704, 2016

PubMed Abstract: Zika virus (ZIKV), a mosquito-borne flavivirus, is a current global public health concern. The flavivirus envelope (E) glycoprotein is responsible for virus entry and represents a major target of neutralizing antibodies for other flaviviruses. Here, we report the structures of ZIKV E protein at 2.0 Å and in complex with a flavivirus broadly neutralizing murine antibody 2A10G6 at 3.0 Å. ZIKV-E resembles all the known flavivirus E structures but contains a unique, positively charged patch adjacent to the fusion loop region of the juxtaposed monomer, which may influence host attachment. The ZIKV-E-2A10G6 complex structure reveals antibody recognition of a highly conserved fusion loop. 2A10G6 binds to ZIKV-E with high affinity in vitro and neutralizes currently circulating ZIKV strains in vitro and in mice. The E protein fusion loop epitope represents a potential candidate for therapeutic antibodies against ZIKV.

PubMed: 27158114
DOI: 10.1016/j.chom.2016.04.013

Experimental method

X-RAY DIFFRACTION (3 Å)