5JGP

Crystal structure of the nitrate/nitrite sensor NarQ fragment bound with iodide ions

5JGP の概要

• エントリーDOI: 10.2210/pdb5jgp/pdb
• 関連するPDBエントリー: 5IJI
• 分子名称: Nitrate/nitrite sensor protein NarQ, NITRATE ION, IODIDE ION, ... (4 entities in total)
• 機能のキーワード: membrane protein, sensor, histidine kinase, iodide, transferase, signaling protein
• 由来する生物種: Escherichia coli (strain K12)
• 細胞内の位置: Cell inner membrane ; Multi-pass membrane protein : P27896
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28397.75

構造登録者

Melnikov, I.,Polovinkin, V.,Popov, A.,Gordeliy, V. (登録日: 2016-04-20, 公開日: 2017-05-31, 最終更新日: 2025-10-01)

主引用文献

Melnikov, I.,Polovinkin, V.,Kovalev, K.,Gushchin, I.,Shevtsov, M.,Shevchenko, V.,Mishin, A.,Alekseev, A.,Rodriguez-Valera, F.,Borshchevskiy, V.,Cherezov, V.,Leonard, G.A.,Gordeliy, V.,Popov, A.
Fast iodide-SAD phasing for high-throughput membrane protein structure determination.
Sci Adv, 3:e1602952-e1602952, 2017

PubMed Abstract: We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide-single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins-the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein-coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.

PubMed: 28508075
DOI: 10.1126/sciadv.1602952

実験手法

X-RAY DIFFRACTION (2.7 Å)