5JFT

Zebra Fish Caspase-3

5JFT の概要

• エントリーDOI: 10.2210/pdb5jft/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000238
• 分子名称: Caspase 3, apoptosis-related cysteine protease a, ACE-ASP-GLU-VAL-ASK, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: phage display substrate library, substrate recognition, danio rerio, apoptosis, hydrolase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Danio rerio (Zebrafish); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57632.31

構造登録者

Tucker, M.B.,MacKenzie, S.H.,Maciag, J.J.,Dirscherl, H.,Swartz, P.D.,Yoder, J.A.,Hamilton, P.T.,Clark, A.C. (登録日: 2016-04-19, 公開日: 2016-10-26, 最終更新日: 2024-11-06)

主引用文献

Tucker, M.B.,MacKenzie, S.H.,Maciag, J.J.,Dirscherl Ackerman, H.,Swartz, P.,Yoder, J.A.,Hamilton, P.T.,Clay Clark, A.
Phage display and structural studies reveal plasticity in substrate specificity of caspase-3a from zebrafish.
Protein Sci., 25:2076-2088, 2016

PubMed Abstract: The regulation of caspase-3 enzyme activity is a vital process in cell fate decisions leading to cell differentiation and tissue development or to apoptosis. The zebrafish, Danio rerio, has become an increasingly popular animal model to study several human diseases because of their transparent embryos, short reproductive cycles, and ease of drug administration. While apoptosis is an evolutionarily conserved process in metazoans, little is known about caspases from zebrafish, particularly regarding substrate specificity and allosteric regulation compared to the human caspases. We cloned zebrafish caspase-3a (casp3a) and examined substrate specificity of the recombinant protein, Casp3a, compared to human caspase-3 (CASP3) by utilizing M13 bacteriophage substrate libraries that incorporated either random amino acids at P5-P1' or aspartate fixed at P1. The results show a preference for the tetrapeptide sequence DNLD for both enzymes, but the P4 position of zebrafish Casp3a also accommodates valine equally well. We determined the structure of zebrafish Casp3a to 2.28Å resolution by X-ray crystallography, and when combined with molecular dynamics simulations, the results suggest that a limited number of amino acid substitutions near the active site result in plasticity of the S4 sub-site by increasing flexibility of one active site loop and by affecting hydrogen-bonding with substrate. The data show that zebrafish Casp3a exhibits a broader substrate portfolio, suggesting overlap with the functions of caspase-6 in zebrafish development.

PubMed: 27577093
DOI: 10.1002/pro.3032

実験手法

X-RAY DIFFRACTION (2.28 Å)