5JFC

NADH-dependent Ferredoxin:NADP Oxidoreductase (NfnI) from Pyrococcus furiosus

5JFC の概要

• エントリーDOI: 10.2210/pdb5jfc/pdb
• 関連するPDBエントリー: 5JCA
• 分子名称: NADH-dependent Ferredoxin:NADP Oxidoreductase subunit alpha, NADH-dependent Ferredoxin:NADP Oxidoreductase subunit beta, IRON/SULFUR CLUSTER, ... (7 entities in total)
• 機能のキーワード: nfni, oxidoreductase, pyrococcus furiosus, nadp(h) bound nfni
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86828.41

構造登録者

Zadvornyy, O.A.,Schut, G.J.,Nguyen, D.M.,Artz, J.H.,Tokmina-Lukaszewska, M.,Lipscomb, G.,King, P.W.,Adams, M.W.,Peters, J.W. (登録日: 2016-04-19, 公開日: 2017-04-12, 最終更新日: 2024-03-06)

主引用文献

Lubner, C.E.,Jennings, D.P.,Mulder, D.W.,Schut, G.J.,Zadvornyy, O.A.,Hoben, J.P.,Tokmina-Lukaszewska, M.,Berry, L.,Nguyen, D.M.,Lipscomb, G.L.,Bothner, B.,Jones, A.K.,Miller, A.F.,King, P.W.,Adams, M.W.W.,Peters, J.W.
Mechanistic insights into energy conservation by flavin-based electron bifurcation.
Nat. Chem. Biol., 13:655-659, 2017

PubMed Abstract: The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism of coordinated coupling of exergonic and endergonic oxidation-reduction reactions by a single enzyme complex has been elucidated through optical and paramagnetic spectroscopic studies revealing unprecedented features. Pairs of electrons are bifurcated over more than 1 volt of electrochemical potential by generating a low-potential, highly energetic, unstable flavin semiquinone and directing electron flow to an iron-sulfur cluster with a highly negative potential to overcome the barrier of the endergonic half reaction. The unprecedented range of thermodynamic driving force that is generated by flavin-based electron bifurcation accounts for unique chemical reactions that are catalyzed by these enzymes.

PubMed: 28394885
DOI: 10.1038/nchembio.2348

実験手法

X-RAY DIFFRACTION (1.598 Å)