5J8Q

Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis

5J8Q の概要

• エントリーDOI: 10.2210/pdb5j8q/pdb
• 分子名称: Cysteine desulfurase SufS, PYRIDOXAL-5'-PHOSPHATE, ALANINE, ... (4 entities in total)
• 機能のキーワード: cysteine desulfurase, iron sulfur cluster, suf-system, transferase
• 由来する生物種: Bacillus subtilis (strain 168)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46316.13

構造登録者

Altegoer, F.,Bange, G. (登録日: 2016-04-08, 公開日: 2016-07-27, 最終更新日: 2024-01-10)

主引用文献

Blauenburg, B.,Mielcarek, A.,Altegoer, F.,Fage, C.D.,Linne, U.,Bange, G.,Marahiel, M.A.
Crystal Structure of Bacillus subtilis Cysteine Desulfurase SufS and Its Dynamic Interaction with Frataxin and Scaffold Protein SufU.
Plos One, 11:e0158749-e0158749, 2016

PubMed Abstract: The biosynthesis of iron sulfur (Fe-S) clusters in Bacillus subtilis is mediated by a SUF-type gene cluster, consisting of the cysteine desulfurase SufS, the scaffold protein SufU, and the putative chaperone complex SufB/SufC/SufD. Here, we present the high-resolution crystal structure of the SufS homodimer in its product-bound state (i.e., in complex with pyrodoxal-5'-phosphate, alanine, Cys361-persulfide). By performing hydrogen/deuterium exchange (H/DX) experiments, we characterized the interaction of SufS with SufU and demonstrate that SufU induces an opening of the active site pocket of SufS. Recent data indicate that frataxin could be involved in Fe-S cluster biosynthesis by facilitating iron incorporation. H/DX experiments show that frataxin indeed interacts with the SufS/SufU complex at the active site. Our findings deepen the current understanding of Fe-S cluster biosynthesis, a complex yet essential process, in the model organism B. subtilis.

PubMed: 27382962
DOI: 10.1371/journal.pone.0158749

実験手法

X-RAY DIFFRACTION (1.702 Å)