5J7A

Bacteriorhodopsin ground state structure obtained with Serial Femtosecond Crystallography

5J7A の概要

• エントリーDOI: 10.2210/pdb5j7a/pdb
• 分子名称: Bacteriorhodopsin, RETINAL, 1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL, ... (4 entities in total)
• 機能のキーワード: light-driven proton pump, retinal binding, seven transmembrane helix protein, proton transport
• 由来する生物種: Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28341.51

構造登録者

Nogly, P.,Panneels, V.,Nelson, G.,Gati, C.,Kimura, T.,Milne, C.,Milathianaki, D.,Kubo, M.,Wu, W.,Conrad, C.,Coe, J.,Bean, R.,Zhao, Y.,Bath, P.,Dods, R.,Harimoorthy, R.,Beyerlein, K.R.,Rheinberger, J.,James, D.,DePonte, D.,Li, C.,Sala, L.,Williams, G.,Hunter, M.,Koglin, J.E.,Berntsen, P.,Nango, E.,Iwata, S.,Chapman, H.N.,Fromme, P.,Frank, M.,Abela, R.,Boutet, S.,Barty, A.,White, T.A.,Weierstall, U.,Spence, J.,Neutze, R.,Schertler, G.,Standfuss, J. (登録日: 2016-04-06, 公開日: 2016-08-31, 最終更新日: 2024-11-13)

主引用文献

Nogly, P.,Panneels, V.,Nelson, G.,Gati, C.,Kimura, T.,Milne, C.,Milathianaki, D.,Kubo, M.,Wu, W.,Conrad, C.,Coe, J.,Bean, R.,Zhao, Y.,Bath, P.,Dods, R.,Harimoorthy, R.,Beyerlein, K.R.,Rheinberger, J.,James, D.,DePonte, D.,Li, C.,Sala, L.,Williams, G.J.,Hunter, M.S.,Koglin, J.E.,Berntsen, P.,Nango, E.,Iwata, S.,Chapman, H.N.,Fromme, P.,Frank, M.,Abela, R.,Boutet, S.,Barty, A.,White, T.A.,Weierstall, U.,Spence, J.,Neutze, R.,Schertler, G.,Standfuss, J.
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.
Nat Commun, 7:12314-12314, 2016

PubMed Abstract: Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

PubMed: 27545823
DOI: 10.1038/ncomms12314

実験手法

X-RAY DIFFRACTION (2.3 Å)