5J63

Crystal Structure of the N-terminal N-formyltransferase Domain (residues 1-306) of Escherichia coli Arna in Complex with UDP-Ara4N and Folinic Acid

5J63 の概要

• エントリーDOI: 10.2210/pdb5j63/pdb
• 分子名称: Bifunctional polymyxin resistance protein ArnA, (2R,3R,4S,5S)-5-amino-3,4-dihydroxytetrahydro-2H-pyran-2-yl [(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate, (4aS,6S)-2-amino-6-{(E)-[(4-methylphenyl)imino]methyl}-4-oxo-4,6,7,8-tetrahydropteridine-5(4aH)-carbaldehyde, ... (4 entities in total)
• 機能のキーワード: lipopolysaccharide, transferase
• 由来する生物種: Escherichia coli H736
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 137503.56

構造登録者

Thoden, J.B.,Genthe, N.A.,Holden, H.M. (登録日: 2016-04-04, 公開日: 2016-05-25, 最終更新日: 2023-09-27)

主引用文献

Genthe, N.A.,Thoden, J.B.,Holden, H.M.
Structure of the Escherichia coli ArnA N-formyltransferase domain in complex with N(5) -formyltetrahydrofolate and UDP-Ara4N.
Protein Sci., 25:1555-1562, 2016

PubMed Abstract: ArnA from Escherichia coli is a key enzyme involved in the formation of 4-amino-4-deoxy-l-arabinose. The addition of this sugar to the lipid A moiety of the lipopolysaccharide of pathogenic Gram-negative bacteria allows these organisms to evade the cationic antimicrobial peptides of the host immune system. Indeed, it is thought that such modifications may be responsible for the repeated infections of cystic fibrosis patients with Pseudomonas aeruginosa. ArnA is a bifunctional enzyme with the N- and C-terminal domains catalyzing formylation and oxidative decarboxylation reactions, respectively. The catalytically competent cofactor for the formylation reaction is N(10) -formyltetrahydrofolate. Here we describe the structure of the isolated N-terminal domain of ArnA in complex with its UDP-sugar substrate and N(5) -formyltetrahydrofolate. The model presented herein may prove valuable in the development of new antimicrobial therapeutics.

PubMed: 27171345
DOI: 10.1002/pro.2938

実験手法

X-RAY DIFFRACTION (2.5 Å)