5J4C

Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution

This is a large structure.

Summary for 5J4C

Related5J4B
Descriptor23S ribosomal RNA, 5S ribosomal RNA, 50S ribosomal protein L2, ... (61 entities in total)
Functional Keywordsribosome, cisplatin, anticancer drug, cytotoxicity, inhibition of translation, translocation, mrna channel, gtpase activating center
Biological sourceEscherichia coli
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Total number of polymer chains112
Total molecular weight4579491.15
Authors
Melnikov, S.V.,Soll, D.,Steitz, T.A.,Polikanov, Y.S. (deposition date: 2016-03-31, release date: 2016-04-27, Last modification date: 2017-09-13)
Primary citation
Melnikov, S.V.,Soll, D.,Steitz, T.A.,Polikanov, Y.S.
Insights into RNA binding by the anticancer drug cisplatin from the crystal structure of cisplatin-modified ribosome.
Nucleic Acids Res., 44:4978-4987, 2016
PubMed: 27079977 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkw246
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.26880.8%2.3%4.7%0.55MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5j4c
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Molmil generated image of 5j4c
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