5IW7

Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor

5IW7 の概要

• エントリーDOI: 10.2210/pdb5iw7/pdb
• EMDBエントリー: 1922 1926 1927
• 分子名称: Ribosome biogenesis protein TSR1,Ribosome biogenesis protein TSR1 (1 entity in total)
• 機能のキーワード: translational gtpase ribosome synthesis, translation
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: Q07381
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 324075.97

構造登録者

McCaughan, U.M.,Jayachandran, U.,Cook, A.G. (登録日: 2016-03-22, 公開日: 2016-06-15, 最終更新日: 2024-05-08)

主引用文献

McCaughan, U.M.,Jayachandran, U.,Shchepachev, V.,Chen, Z.A.,Rappsilber, J.,Tollervey, D.,Cook, A.G.
Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases.
Nat Commun, 7:11789-11789, 2016

PubMed Abstract: Budding yeast Tsr1 is a ribosome biogenesis factor with sequence similarity to GTPases, which is essential for cytoplasmic steps in 40S subunit maturation. Here we present the crystal structure of Tsr1 at 3.6 Å. Tsr1 has a similar domain architecture to translational GTPases such as EF-Tu and the selenocysteine incorporation factor SelB. However, active site residues required for GTP binding and hydrolysis are absent, explaining the lack of enzymatic activity in previous analyses. Modelling of Tsr1 into cryo-electron microscopy maps of pre-40S particles shows that a highly acidic surface of Tsr1 is presented on the outside of pre-40S particles, potentially preventing premature binding to 60S subunits. Late pre-40S maturation also requires the GTPase eIF5B and the ATPase Rio1. The location of Tsr1 is predicted to block binding by both factors, strongly indicating that removal of Tsr1 is an essential step during cytoplasmic maturation of 40S ribosomal subunits.

PubMed: 27250689
DOI: 10.1038/ncomms11789

実験手法

X-RAY DIFFRACTION (3.6 Å)