5IUW

Crystal Structure of Indole-3-acetaldehyde Dehydrogenase in complexed with NAD+ and IAA

5IUW の概要

• エントリーDOI: 10.2210/pdb5iuw/pdb
• 関連するPDBエントリー: 5IUU 5IUV
• 分子名称: Aldehyde dehydrogenase family protein, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, 1H-INDOL-3-YLACETIC ACID, ... (4 entities in total)
• 機能のキーワード: indole-3-acetaldehyde dehydrogenase, aldehyde dehydrogenase, oxidoreductase
• 由来する生物種: Pseudomonas syringae pv. tomato (strain DC3000)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 107236.87

構造登録者

Lee, S.G.,McClerklin, S.,Kunkel, B.,Jez, J.M. (登録日: 2016-03-18, 公開日: 2017-10-25, 最終更新日: 2023-09-27)

主引用文献

McClerklin, S.A.,Lee, S.G.,Harper, C.P.,Nwumeh, R.,Jez, J.M.,Kunkel, B.N.
Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000.
PLoS Pathog., 14:e1006811-e1006811, 2018

PubMed Abstract: The bacterial pathogen Pseudomonas syringae modulates plant hormone signaling to promote infection and disease development. P. syringae uses several strategies to manipulate auxin physiology in Arabidopsis thaliana to promote pathogenesis, including its synthesis of indole-3-acetic acid (IAA), the predominant form of auxin in plants, and production of virulence factors that alter auxin responses in the host; however, the role of pathogen-derived auxin in P. syringae pathogenesis is not well understood. Here we demonstrate that P. syringae strain DC3000 produces IAA via a previously uncharacterized pathway and identify a novel indole-3-acetaldehyde dehydrogenase, AldA, that functions in IAA biosynthesis by catalyzing the NAD-dependent formation of IAA from indole-3-acetaldehyde (IAAld). Biochemical analysis and solving of the 1.9 Å resolution x-ray crystal structure reveal key features of AldA for IAA synthesis, including the molecular basis of substrate specificity. Disruption of aldA and a close homolog, aldB, lead to reduced IAA production in culture and reduced virulence on A. thaliana. We use these mutants to explore the mechanism by which pathogen-derived auxin contributes to virulence and show that IAA produced by DC3000 suppresses salicylic acid-mediated defenses in A. thaliana. Thus, auxin is a DC3000 virulence factor that promotes pathogenicity by suppressing host defenses.

PubMed: 29293681
DOI: 10.1371/journal.ppat.1006811

実験手法

X-RAY DIFFRACTION (2.093 Å)