5IU0
Rubisco from Arabidopsis thaliana
Summary for 5IU0
| Entry DOI | 10.2210/pdb5iu0/pdb |
| Descriptor | Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain 1B, chloroplastic, MAGNESIUM ION, ... (6 entities in total) |
| Functional Keywords | ribulose-bisphosphate-carboxylase, arabidopsis thaliana, lyase |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) More |
| Total number of polymer chains | 4 |
| Total formula weight | 148312.01 |
| Authors | Valegaard, K.,Hasse, D.,Gunn, L.,Andersson, I. (deposition date: 2016-03-17, release date: 2017-07-12, Last modification date: 2024-01-10) |
| Primary citation | Valegard, K.,Hasse, D.,Andersson, I.,Gunn, L.H. Structure of Rubisco from Arabidopsis thaliana in complex with 2-carboxyarabinitol-1,5-bisphosphate. Acta Crystallogr D Struct Biol, 74:1-9, 2018 Cited by PubMed Abstract: The crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Arabidopsis thaliana is reported at 1.5 Å resolution. In light of the importance of A. thaliana as a model organism for understanding higher plant biology, and the pivotal role of Rubisco in photosynthetic carbon assimilation, there has been a notable absence of an A. thaliana Rubisco crystal structure. A. thaliana Rubisco is an LS hexadecamer comprising eight plastome-encoded catalytic large (L) subunits and eight nuclear-encoded small (S) subunits. A. thaliana produces four distinct small-subunit isoforms (RbcS1A, RbcS1B, RbcS2B and RbcS3B), and this crystal structure provides a snapshot of A. thaliana Rubisco containing the low-abundance RbcS3B small-subunit isoform. Crystals were obtained in the presence of the transition-state analogue 2-carboxy-D-arabinitol-1,5-bisphosphate. A. thaliana Rubisco shares the overall fold characteristic of higher plant Rubiscos, but exhibits an interesting disparity between sequence and structural relatedness to other Rubisco isoforms. These results provide the structural framework to understand A. thaliana Rubisco and the potential catalytic differences that could be conferred by alternative A. thaliana Rubisco small-subunit isoforms. PubMed: 29372894DOI: 10.1107/S2059798317017132 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.499 Å) |
Structure validation
Download full validation report
