5IU0

Rubisco from Arabidopsis thaliana

5IU0 の概要

• エントリーDOI: 10.2210/pdb5iu0/pdb
• 分子名称: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain 1B, chloroplastic, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: ribulose-bisphosphate-carboxylase, arabidopsis thaliana, lyase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 148312.01

構造登録者

Valegaard, K.,Hasse, D.,Gunn, L.,Andersson, I. (登録日: 2016-03-17, 公開日: 2017-07-12, 最終更新日: 2024-01-10)

主引用文献

Valegard, K.,Hasse, D.,Andersson, I.,Gunn, L.H.
Structure of Rubisco from Arabidopsis thaliana in complex with 2-carboxyarabinitol-1,5-bisphosphate.
Acta Crystallogr D Struct Biol, 74:1-9, 2018

PubMed Abstract: The crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Arabidopsis thaliana is reported at 1.5 Å resolution. In light of the importance of A. thaliana as a model organism for understanding higher plant biology, and the pivotal role of Rubisco in photosynthetic carbon assimilation, there has been a notable absence of an A. thaliana Rubisco crystal structure. A. thaliana Rubisco is an LS hexadecamer comprising eight plastome-encoded catalytic large (L) subunits and eight nuclear-encoded small (S) subunits. A. thaliana produces four distinct small-subunit isoforms (RbcS1A, RbcS1B, RbcS2B and RbcS3B), and this crystal structure provides a snapshot of A. thaliana Rubisco containing the low-abundance RbcS3B small-subunit isoform. Crystals were obtained in the presence of the transition-state analogue 2-carboxy-D-arabinitol-1,5-bisphosphate. A. thaliana Rubisco shares the overall fold characteristic of higher plant Rubiscos, but exhibits an interesting disparity between sequence and structural relatedness to other Rubisco isoforms. These results provide the structural framework to understand A. thaliana Rubisco and the potential catalytic differences that could be conferred by alternative A. thaliana Rubisco small-subunit isoforms.

PubMed: 29372894
DOI: 10.1107/S2059798317017132

実験手法

X-RAY DIFFRACTION (1.499 Å)