5ITP

Structure of the periplasmic binding protein NocT from A.tumefaciens in complex with octopine

5ITP の概要

• エントリーDOI: 10.2210/pdb5itp/pdb
• 関連するPDBエントリー: 4p0i
• 分子名称: Nopaline-binding periplasmic protein, octopine, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: periplasmic binding protein, opine, membrane protein
• 由来する生物種: Agrobacterium fabrum
• 細胞内の位置: Periplasm : P35120
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59552.38

構造登録者

Vigouroux, A.,Morera, S. (登録日: 2016-03-17, 公開日: 2016-11-30, 最終更新日: 2024-01-10)

主引用文献

Lang, J.,Vigouroux, A.,El Sahili, A.,Kwasiborski, A.,Aumont-Nicaise, M.,Dessaux, Y.,Shykoff, J.A.,Morera, S.,Faure, D.
Fitness costs restrict niche expansion by generalist niche-constructing pathogens.
ISME J, 11:374-385, 2017

PubMed Abstract: We investigated the molecular and ecological mechanisms involved in niche expansion, or generalism, versus specialization in sympatric plant pathogens. Nopaline-type and octopine-type Agrobacterium tumefaciens engineer distinct niches in their plant hosts that provide different nutrients: nopaline or octopine, respectively. Previous studies revealed that nopaline-type pathogens may expand their niche to also assimilate octopine in the presence of nopaline, but consequences of this phenomenon on pathogen dynamics in planta were not known. Here, we provided molecular insight into how the transport protein NocT can bind octopine as well as nopaline, contributing to niche expansion. We further showed that despite the ability for niche expansion, nopaline-type pathogens had no competitive advantage over octopine-type pathogens in co-infected plants. We also demonstrated that a single nucleotide polymorphism in the nocR gene was sufficient to allow octopine assimilation by nopaline-type strains even in absence of nopaline. The evolved nocR bacteria had higher fitness than their ancestor in octopine-rich transgenic plants but lower fitness in tumors induced by octopine-type pathogens. Overall, this work elucidates the specialization of A. tumefaciens to particular opine niches and explains why generalists do not always spread despite the advantage associated with broader nutritional niches.

PubMed: 27801902
DOI: 10.1038/ismej.2016.137

実験手法

X-RAY DIFFRACTION (1.85 Å)