5IQZ

Crystal structure of N-terminal domain of Human SIRT7

Summary for 5IQZ

• Entry DOI: 10.2210/pdb5iqz/pdb
• Related PRD ID: PRD_900001
• Descriptor: SIRT7 protein, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose (3 entities in total)
• Functional Keywords: sirt7, fusion protein, maltose binding, alpha histone deacetylase sirtuin, sugar binding protein
• Biological source: Homo sapiens
• Total number of polymer chains: 1
• Total formula weight: 49506.05

Authors

Thakur, K.G.,Priyanka, A. (deposition date: 2016-03-11, release date: 2016-07-27, Last modification date: 2023-11-08)

Primary citation

Priyanka, A.,Solanki, V.,Parkesh, R.,Thakur, K.G.
Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture
Proteins, 84:1558-1563, 2016

PubMed Abstract: Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 Å resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563. © 2016 Wiley Periodicals, Inc.

PubMed: 27287224
DOI: 10.1002/prot.25085

Experimental method

X-RAY DIFFRACTION (2.334 Å)