5IOL

Crystal structure of Nucleoside Diphosphate Kinase from Schistosoma mansoni

5IOL の概要

• エントリーDOI: 10.2210/pdb5iol/pdb
• 関連するPDBエントリー: 5IOM
• 分子名称: Nucleoside diphosphate kinase (2 entities in total)
• 機能のキーワード: nucleoside diphosphate kinase, enzyme, transferase
• 由来する生物種: Schistosoma mansoni (Blood fluke)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 202744.31

構造登録者

Torini, J.R.S.,Romanello, L.,Bird, L.E.,Nettleship, J.E.,Owens, R.J.,Aller, P.,DeMarco, R.,Brandao-Neto, J.,Pereira, H.M. (登録日: 2016-03-08, 公開日: 2017-03-08, 最終更新日: 2023-09-27)

主引用文献

Torini, J.R.,de Freitas Fernandes, A.,Balasco Serrao, V.H.,Romanello, L.,Bird, L.E.,Nettleship, J.E.,Owens, R.J.,Brandao-Neto, J.,Zeraik, A.E.,DeMarco, R.,D'Muniz Pereira, H.
Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs.
Mol.Biochem.Parasitol., :111187-111187, 2019

PubMed Abstract: Nucleoside diphosphate kinases (NDPKs) are crucial to keep the high triphosphate nucleotide levels in the biological process. The enzymatic mechanism has been extensively described; however, the structural characteristics and kinetic parameters have never been fully determined. In Schistosoma mansoni, NDPK (SmNDPK) is directly involved in the pyrimidine and purine salvage pathways, being essential for nucleotide metabolism. The SmNDPK enzymatic activity is the highest of the known purine metabolisms when compared to the mammalian NDPKs, suggesting the importance of this enzyme in the worm metabolism. Here, we report the recombinant expression of SmNDPK that resulted in 1.7 and 1.9 Å apo-form structure in different space-groups, as well as the 2.1 Å SmNDPK.ADP complex. The binding and kinetic assays reveal the ATP-dependence for enzyme activation. Moreover, in situ hybridization showed that SmNDPK transcripts are found in reproductive organs and in the esophagus gland of adult worms, which can be intrinsically related with the oviposition and digestive processes. These results will help us fully understand the crucial participation of this enzyme in Schistosoma mansoni and its importance for the pathology of the disease.

PubMed: 31103556
DOI: 10.1016/j.molbiopara.2019.111187

実験手法

X-RAY DIFFRACTION (1.741 Å)