5INE

Crystal structure of the prefusion glycoprotein of LCMV

5INE の概要

• エントリーDOI: 10.2210/pdb5ine/pdb
• 分子名称: Pre-glycoprotein polyprotein GP complex, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: arenavirus, lcmv, glycoprotein, pre-fusion, viral protein
• 由来する生物種: Lymphocytic choriomeningitis mammarenavirus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 107866.16

構造登録者

Hastie, K.M.,Saphire, E.O. (登録日: 2016-03-07, 公開日: 2016-04-20, 最終更新日: 2024-10-23)

主引用文献

Hastie, K.M.,Igonet, S.,Sullivan, B.M.,Legrand, P.,Zandonatti, M.A.,Robinson, J.E.,Garry, R.F.,Rey, F.A.,Oldstone, M.B.,Saphire, E.O.
Crystal structure of the prefusion surface glycoprotein of the prototypic arenavirus LCMV.
Nat.Struct.Mol.Biol., 23:513-521, 2016

PubMed Abstract: Arenaviruses exist worldwide and can cause hemorrhagic fever and neurologic disease. A single glycoprotein expressed on the viral surface mediates entry into target cells. This glycoprotein, termed GPC, contains a membrane-associated signal peptide, a receptor-binding subunit termed GP1 and a fusion-mediating subunit termed GP2. Although GPC is a critical target of antibodies and vaccines, the structure of the metastable GP1-GP2 prefusion complex has remained elusive for all arenaviruses. Here we describe the crystal structure of the fully glycosylated prefusion GP1-GP2 complex of the prototypic arenavirus LCMV at 3.5 Å. This structure reveals the conformational changes that the arenavirus glycoprotein must undergo to cause fusion and illustrates the fusion regions and potential oligomeric states.

PubMed: 27111888
DOI: 10.1038/nsmb.3210

実験手法

X-RAY DIFFRACTION (3.5 Å)