5ILC

The X-ray structure of the adduct formed in the reaction between hen egg white lysozyme a compound 2, a platin(II) compound containing a O, S bidentate ligand

5ILC の概要

• エントリーDOI: 10.2210/pdb5ilc/pdb
• 分子名称: Lysozyme C, PLATINUM (II) ION, DIMETHYL SULFOXIDE, ... (7 entities in total)
• 機能のキーワード: metallodrug, protein platination, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14863.67

構造登録者

Merlino, A.,Ferraro, G. (登録日: 2016-03-04, 公開日: 2016-12-07, 最終更新日: 2024-11-13)

主引用文献

Hildebrandt, J.,Hafner, N.,Gorls, H.,Kritsch, D.,Ferraro, G.,Durst, M.,Runnebaum, I.B.,Merlino, A.,Weigand, W.
Platinum(ii) O,S complexes as potential metallodrugs against Cisplatin resistance.
Dalton Trans, 45:18876-18891, 2016

PubMed Abstract: We report on platinum(ii) complexes with different cinnamic acid derivatives as ligands with cytotoxic activity against Cisplatin resistant ovarian cancer cell line subcultures of SKOV3 and A2780. A typical mechanism of action for platinum(ii) complexes as Cisplatin itself is binding to the DNA and inducing double-strand breaks. We examined the biological behavior of these potential drugs with 9-methylguanine using NMR spectroscopic methods and their DNA damage potential including γH2AX-foci analyses. X-ray diffraction methods have been used to elucidate the molecular structures of the platinum(ii) complexes. Interactions with the model protein lysozyme have been evaluated by different techniques including UV-Vis absorption spectroscopy, fluorescence and X-ray crystallography.

PubMed: 27897281
DOI: 10.1039/c6dt01388k

実験手法

X-RAY DIFFRACTION (1.75 Å)