5IJO

Alternative composite structure of the inner ring of the human nuclear pore complex (16 copies of Nup188, 16 copies of Nup205)

Summary for 5IJO

• Entry DOI: 10.2210/pdb5ijo/pdb
• EMDB information: 8085
• Descriptor: Nuclear pore complex protein Nup155, Nuclear pore complex protein Nup93, Nuclear pore complex protein Nup205, ... (7 entities in total)
• Functional Keywords: nuclear pore complex, nucleocytoplasmic transport, transport protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 26
• Total formula weight: 2834288.06

Authors

Kosinski, J.,Mosalaganti, S.,von Appen, A.,Beck, M. (deposition date: 2016-03-02, release date: 2016-04-27, Last modification date: 2024-05-08)

Primary citation

Kosinski, J.,Mosalaganti, S.,von Appen, A.,Teimer, R.,DiGuilio, A.L.,Wan, W.,Bui, K.H.,Hagen, W.J.,Briggs, J.A.,Glavy, J.S.,Hurt, E.,Beck, M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science, 352:363-365, 2016

PubMed Abstract: Nuclear pore complexes (NPCs) are 110-megadalton assemblies that mediate nucleocytoplasmic transport. NPCs are built from multiple copies of ~30 different nucleoporins, and understanding how these nucleoporins assemble into the NPC scaffold imposes a formidable challenge. Recently, it has been shown how the Y complex, a prominent NPC module, forms the outer rings of the nuclear pore. However, the organization of the inner ring has remained unknown until now. We used molecular modeling combined with cross-linking mass spectrometry and cryo-electron tomography to obtain a composite structure of the inner ring. This architectural map explains the vast majority of the electron density of the scaffold. We conclude that despite obvious differences in morphology and composition, the higher-order structure of the inner and outer rings is unexpectedly similar.

PubMed: 27081072
DOI: 10.1126/science.aaf0643

Experimental method

ELECTRON MICROSCOPY (21.4 Å)