5ICN

HDAC1:MTA1 in complex with inositol-6-phosphate and a novel peptide inhibitor based on histone H4

5ICN の概要

• エントリーDOI: 10.2210/pdb5icn/pdb
• 分子名称: Metastasis-associated protein MTA1, Histone deacetylase 1, GLY-ALA-6A0-ARG-HIS, ... (6 entities in total)
• 機能のキーワード: transcription repression inositol phosphate corepressor histone deacetylase hdac1 hdac3 histones, transcription, metastasis associated protein, ip6
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67157.56

構造登録者

Millard, C.J.,Robertson, N.S.,Watson, P.J.,Jameson, A.G.,Schwabe, J.W.R. (登録日: 2016-02-23, 公開日: 2016-05-11, 最終更新日: 2024-10-16)

主引用文献

Watson, P.J.,Millard, C.J.,Riley, A.M.,Robertson, N.S.,Wright, L.C.,Godage, H.Y.,Cowley, S.M.,Jamieson, A.G.,Potter, B.V.,Schwabe, J.W.
Insights into the activation mechanism of class I HDAC complexes by inositol phosphates.
Nat Commun, 7:11262-11262, 2016

PubMed Abstract: Histone deacetylases (HDACs) 1, 2 and 3 form the catalytic subunit of several large transcriptional repression complexes. Unexpectedly, the enzymatic activity of HDACs in these complexes has been shown to be regulated by inositol phosphates, which bind in a pocket sandwiched between the HDAC and co-repressor proteins. However, the actual mechanism of activation remains poorly understood. Here we have elucidated the stereochemical requirements for binding and activation by inositol phosphates, demonstrating that activation requires three adjacent phosphate groups and that other positions on the inositol ring can tolerate bulky substituents. We also demonstrate that there is allosteric communication between the inositol-binding site and the active site. The crystal structure of the HDAC1:MTA1 complex bound to a novel peptide-based inhibitor and to inositol hexaphosphate suggests a molecular basis of substrate recognition, and an entropically driven allosteric mechanism of activation.

PubMed: 27109927
DOI: 10.1038/ncomms11262

実験手法

X-RAY DIFFRACTION (3.3 Å)