5I9S

MicroED structure of proteinase K at 1.75 A resolution

5I9S の概要

• エントリーDOI: 10.2210/pdb5i9s/pdb
• EMDBエントリー: 8077
• 分子名称: Proteinase K, SULFATE ION (3 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Engyodontium album
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29150.92

構造登録者

Hattne, J.,Shi, D.,de la Cruz, M.J.,Reyes, F.E.,Gonen, T. (登録日: 2016-02-20, 公開日: 2016-06-08, 最終更新日: 2024-11-13)

主引用文献

Hattne, J.,Shi, D.,de la Cruz, M.J.,Reyes, F.E.,Gonen, T.
Modeling truncated pixel values of faint reflections in MicroED images.
J.Appl.Crystallogr., 49:1029-1034, 2016

PubMed Abstract: The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images.

PubMed: 27275145
DOI: 10.1107/S1600576716007196

実験手法

ELECTRON CRYSTALLOGRAPHY (1.75 Å)