5I9D

Crystal structure of designed pentatricopeptide repeat protein dPPR-U8A2 in complex with its target RNA U8A2

5I9D の概要

• エントリーDOI: 10.2210/pdb5i9d/pdb
• 関連するPDBエントリー: 5I9F 5I9G 5I9H
• 分子名称: pentatricopeptide repeat protein dPPR-U8A2, RNA (5'-R(*GP*GP*GP*G*UP*UP*UP*UP*AP*AP*UP*UP*UP*UP*CP*CP*CP*C)-3') (3 entities in total)
• 機能のキーワード: rna binding protein, single-stranded rna, designer pentatricopeptide repeat protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: unidentified; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57126.94

構造登録者

Shen, C.,Zhang, D.,Guan, Z.,Zou, T.,Yin, P. (登録日: 2016-02-20, 公開日: 2016-04-27, 最終更新日: 2023-11-08)

主引用文献

Shen, C.,Zhang, D.,Guan, Z.,Liu, Y.,Yang, Z.,Yang, Y.,Wang, X.,Wang, Q.,Zhang, Q.,Fan, S.,Zou, T.,Yin, P.
Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins.
Nat Commun, 7:11285-11285, 2016

PubMed Abstract: As a large family of RNA-binding proteins, pentatricopeptide repeat (PPR) proteins mediate multiple aspects of RNA metabolism in eukaryotes. Binding to their target single-stranded RNAs (ssRNAs) in a modular and base-specific fashion, PPR proteins can serve as designable modules for gene manipulation. However, the structural basis for nucleotide-specific recognition by designer PPR (dPPR) proteins remains to be elucidated. Here, we report four crystal structures of dPPR proteins in complex with their respective ssRNA targets. The dPPR repeats are assembled into a right-handed superhelical spiral shell that embraces the ssRNA. Interactions between different PPR codes and RNA bases are observed at the atomic level, revealing the molecular basis for the modular and specific recognition patterns of the RNA bases U, C, A and G. These structures not only provide insights into the functional study of PPR proteins but also open a path towards the potential design of synthetic sequence-specific RNA-binding proteins.

PubMed: 27088764
DOI: 10.1038/ncomms11285

実験手法

X-RAY DIFFRACTION (2.596 Å)