5I30

Crystal structure of the human astrovirus 2 neutralizing monoclonal antibody PL-2 Fab fragment at 1.9 A resolution

5I30 の概要

• エントリーDOI: 10.2210/pdb5i30/pdb
• 分子名称: Fab PL-2 light chain, Fab PL-2 heavy chain, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: antibody fab fragment, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47475.59

構造登録者

Bogdanoff, W.A.,DuBois, R.M. (登録日: 2016-02-09, 公開日: 2016-04-13, 最終更新日: 2024-10-23)

主引用文献

Bogdanoff, W.A.,Morgenstern, D.,Bern, M.,Ueberheide, B.M.,Sanchez-Fauquier, A.,DuBois, R.M.
De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.
Acs Infect Dis., 2:313-321, 2016

PubMed Abstract: Monoclonal antibody (mAb) therapeutics targeting cancer, autoimmune diseases, inflammatory diseases, and infectious diseases are growing exponentially. Although numerous panels of mAbs targeting infectious disease agents have been developed, their progression into clinically useful mAbs is often hindered by the lack of sequence information and/or loss of hybridoma cells that produce them. Here we combine the power of crystallography and mass spectrometry to determine the amino acid sequence and glycosylation modification of the Fab fragment of a potent human astrovirus-neutralizing mAb. We used this information to engineer a recombinant antibody single-chain variable fragment that has the same specificity as the parent monoclonal antibody to bind to the astrovirus capsid protein. This antibody can now potentially be developed as a therapeutic and diagnostic agent.

PubMed: 27213181
DOI: 10.1021/acsinfecdis.6b00026

実験手法

X-RAY DIFFRACTION (1.9 Å)