5HYD

Crystal structure of calcium-free human S100Z

5HYD の概要

• エントリーDOI: 10.2210/pdb5hyd/pdb
• 関連するPDBエントリー: 2WCE 2Y5I
• 分子名称: Protein S100-Z (2 entities in total)
• 機能のキーワード: s100, calcium-promoted oligomerisation neuronal development, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 46550.37

構造登録者

Calderone, V.,Fragai, M.,Luchinat, C.,Gallo, G. (登録日: 2016-02-01, 公開日: 2017-02-01, 最終更新日: 2024-11-13)

主引用文献

Calderone, V.,Fragai, M.,Gallo, G.,Luchinat, C.
Solving the crystal structure of human calcium-free S100Z: the siege and conquer of one of the last S100 family strongholds.
J. Biol. Inorg. Chem., 22:519-526, 2017

PubMed Abstract: The X-ray structure of human apo-S100Z has been solved and compared with that of the zebrafish calcium-bound S100Z, which is the closest in sequence. Human apo-S100A12, which shows only 43% sequence identity to human S100Z, has been used as template model to solve the crystallographic phase problem. Although a significant buried surface area between the two physiological dimers is present in the asymmetric unit of human apo-S100Z, the protein does not form the superhelical arrangement in the crystal as observed for the zebrafish calcium-bound S100Z and human calcium-bound S100A4. These findings further demonstrate that calcium plays a fundamental role in triggering quaternary structure formation in several S100s. Solving the X-ray structure of human apo-S100Z by standard molecular replacement procedures turned out to be a challenge and required trying different models and different software tools among which only one was successful. The model that allowed structure solution was that with one of the lowest sequence identity with the target protein among the S100 family in the apo state. Based on the previously solved zebrafish holo-S100Z, a putative human holo-S100Z structure has been then calculated through homology modeling; the differences between the experimental human apo and calculated holo structure have been compared to those existing for other members of the family.

PubMed: 28074300
DOI: 10.1007/s00775-017-1437-4

実験手法

X-RAY DIFFRACTION (2.3 Å)