5HYC

Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus

Summary for 5HYC

• Entry DOI: 10.2210/pdb5hyc/pdb
• Related: 5HXL
• Descriptor: Uncharacterized protein, Cytoplasmic dynein 1 intermediate chain 2 (3 entities in total)
• Functional Keywords: dynein light chain tctex-1, magnaporthe oryzae, unknown function
• Biological source: Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus); More
• Total number of polymer chains: 3
• Total formula weight: 38950.57

Authors

Liu, J.,Li, G.,Huang, J.,Peng, Y.-l. (deposition date: 2016-02-01, release date: 2017-02-01, Last modification date: 2023-11-08)

Primary citation

Li, G.,Huang, J.,Yang, J.,He, D.,Wang, C.,Qi, X.,Taylor, I.A.,Liu, J.,Peng, Y.L.
Structure based function-annotation of hypothetical protein MGG_01005 from Magnaporthe oryzae reveals it is the dynein light chain orthologue of dynlt1/3.
Sci Rep, 8:3952-3952, 2018

PubMed Abstract: Magnaporthe oryzae is a model fungal plant pathogen employed for studying plant-fungi interactions. Whole genome sequencing and bioinformatics analyses revealed that this fungal pathogen has more than 12,000 protein-coding genes with 65% of the genes remaining functionally un-annotated. Here, we determine the structure of the hypothetical protein, MGG_01005 and show that it is the Magnaporthe oryzae Dynein light chain Tctex-type 1 (dynlt1/3), demonstrated by its structural similarity to other orthologous dynlt1 proteins and its conserved interaction with the N-terminus of the Magnaporthe oryzae dynein intermediate chain, MoDyn1I2. In addition, we present the structure of the MGG_01005-MoDyn1I2 complex together with mutagenesis studies that reveals a di-histidine motif interaction with a glutamate residue in the dynein intermediate chain within a conserved molecular interface. These results demonstrate the utility of structure-based annotation and validate it as a viable approach for the molecular assignment of hypothetic proteins from phyto-pathogenic fungi.

PubMed: 29500373
DOI: 10.1038/s41598-018-21667-5

Experimental method

X-RAY DIFFRACTION (2.4 Å)