5HWZ

Crystal structure of nitrophorin 4 D30N mutant with nitrite

5HWZ の概要

• エントリーDOI: 10.2210/pdb5hwz/pdb
• 分子名称: Nitrophorin-4, PROTOPORPHYRIN IX CONTAINING FE, NITRITE ION, ... (4 entities in total)
• 機能のキーワード: nitrophorin, nitrite, heme, transport protein
• 由来する生物種: Rhodnius prolixus (Triatomid bug)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20954.17

構造登録者

Ogata, H.,He, C.,Lubitz, W. (登録日: 2016-01-29, 公開日: 2016-05-04, 最終更新日: 2024-11-06)

主引用文献

He, C.,Ogata, H.,Lubitz, W.
Elucidation of the heme active site electronic structure affecting the unprecedented nitrite dismutase activity of the ferrihemebproteins, the nitrophorins.
Chem Sci, 7:5332-5340, 2016

PubMed Abstract: Nitrophorins (NPs) catalyze the nitrite dismutation reaction that is unprecedented in ferriheme proteins. Despite progress in studying the reaction mechanism, fundamental issues regarding the correlation of the structural features with the nitrite dismutase activity of NPs remain elusive. On the other hand, it has been shown that the nitrite complexes of NPs are unique among those of the ferriheme proteins since some of their electron paramagnetic resonance (EPR) spectra show significant highly anisotropic low spin (HALS) signals with large values over 3.2. The origin of HALS signals in ferriheme proteins or models is not well understood, especially in cases where axial ligands other than histidine are present. In this study several mutations were introduced in NP4. The related nitrite coordination and dismutation reaction were investigated. As a result, the EPR spectra of the NP-nitrite complexes were found to be tightly correlated with the extent of heme ruffling and protonation state of the proximal His ligand-dictated by an extended H-bonding network at the heme active site. Furthermore, it is established that the two factors are essential in determining the nitrite dismutase activity of NPs. These results may provide a valuable guide for identifying or designing novel heme proteins with similar activity.

PubMed: 30155185
DOI: 10.1039/c6sc01019a

実験手法

X-RAY DIFFRACTION (1.45 Å)