5HNL

In-house X-ray single crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels

Summary for 5HNL

• Entry DOI: 10.2210/pdb5hnl/pdb
• Related: 5HNC
• Descriptor: Lysozyme C (2 entities in total)
• Functional Keywords: hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14331.16

Authors

Tsukui, S.,Kimura, F.,Kusaka, K.,Baba, S.,Mizuno, N.,Kimura, T. (deposition date: 2016-01-18, release date: 2016-07-20, Last modification date: 2024-10-30)

Primary citation

Tsukui, S.,Kimura, F.,Kusaka, K.,Baba, S.,Mizuno, N.,Kimura, T.
Neutron and X-ray single-crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels.
Acta Crystallogr D Struct Biol, 72:823-829, 2016

PubMed Abstract: Protein microcrystals magnetically aligned in D2O hydrogels were subjected to neutron diffraction measurements, and reflections were observed for the first time to a resolution of 3.4 Å from lysozyme microcrystals (∼10 × 10 × 50 µm). This result demonstrated the possibility that magnetically oriented microcrystals consolidated in D2O gels may provide a promising means to obtain single-crystal neutron diffraction from proteins that do not crystallize at the sizes required for neutron diffraction structure determination. In addition, lysozyme microcrystals aligned in H2O hydrogels allowed structure determination at a resolution of 1.76 Å at room temperature by X-ray diffraction. The use of gels has advantages since the microcrystals are measured under hydrated conditions.

PubMed: 27377379
DOI: 10.1107/S2059798316007415

Experimental method

X-RAY DIFFRACTION (2.424 Å)