5HN2

Base Pairing and Structure Insights into the 5-Formylcytosine in RNA Duplex

Summary for 5HN2

• Entry DOI: 10.2210/pdb5hn2/pdb
• Descriptor: RNA (5'-R(*GP*UP*AP*(OFC)P*GP*UP*AP*C)-3'), SODIUM ION (3 entities in total)
• Functional Keywords: rna, 5-formylcytosine
• Biological source: synthetic construct
• Total number of polymer chains: 6
• Total formula weight: 15350.42

Authors

Luo, Z.P.,Sheng, J. (deposition date: 2016-01-18, release date: 2016-04-27, Last modification date: 2023-09-27)

Primary citation

Wang, R.,Luo, Z.,He, K.,Delaney, M.O.,Chen, D.,Sheng, J.
Base pairing and structural insights into the 5-formylcytosine in RNA duplex.
Nucleic Acids Res., 44:4968-4977, 2016

PubMed Abstract: 5-Formylcytidine (f(5)C), a previously discovered natural nucleotide in the mitochondrial tRNA of many species including human, has been recently detected as the oxidative product of 5-methylcytidine (m(5)C) through 5-hydroxymethylcytidine (hm(5)C) in total RNA of mammalian cells. The discovery indicated that these cytosine derivatives in RNA might also play important epigenetic roles similar as in DNA, which has been intensively investigated in the past few years. In this paper, we studied the base pairing specificity of f(5)C in different RNA duplex contexts. We found that the 5-formyl group could increase duplex thermal stability and enhance base pairing specificity. We present three high-resolution crystal structures of an octamer RNA duplex [5'-GUA(f(5)C)GUAC-3']2 that have been solved under three crystallization conditions with different buffers and pH values. Our results showed that the 5-formyl group is located in the same plane as the cytosine base and forms an intra-residue hydrogen bond with the amino group in the N4 position. In addition, this modification increases the base stacking between the f(5)C and the neighboring bases while not causing significant global and local structure perturbations. This work provides insights into the effects of 5-formylcytosine on RNA duplex.

PubMed: 27079978
DOI: 10.1093/nar/gkw235

Experimental method

X-RAY DIFFRACTION (1.5 Å)