5HLZ

Structure of Pro-Activin A Complex at 2.85 A resolution

5HLZ の概要

• エントリーDOI: 10.2210/pdb5hlz/pdb
• 分子名称: Inhibin beta A chain (3 entities in total)
• 機能のキーワード: growth factor, precursor, signalling, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Secreted: P08476 P08476
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 173057.22

構造登録者

Wang, X.,Fischer, G.,Hyvonen, M. (登録日: 2016-01-15, 公開日: 2016-07-13, 最終更新日: 2024-11-06)

主引用文献

Wang, X.,Fischer, G.,Hyvonen, M.
Structure and activation of pro-activin A.
Nat Commun, 7:12052-12052, 2016

PubMed Abstract: Activins are growth factors with multiple roles in the development and homeostasis. Like all TGF-β family of growth factors, activins are synthesized as large precursors from which mature dimeric growth factors are released proteolytically. Here we have studied the activation of activin A and determined crystal structures of the unprocessed precursor and of the cleaved pro-mature complex. Replacing the natural furin cleavage site with a HRV 3C protease site, we show how the protein gains its bioactivity after proteolysis and is as active as the isolated mature domain. The complex remains associated in conditions used for biochemical analysis with a dissociation constant of 5 nM, but the pro-domain can be actively displaced from the complex by follistatin. Our high-resolution structures of pro-activin A share features seen in the pro-TGF-β1 and pro-BMP-9 structures, but reveal a new oligomeric arrangement, with a domain-swapped, cross-armed conformation for the protomers in the dimeric protein.

PubMed: 27373274
DOI: 10.1038/ncomms12052

実験手法

X-RAY DIFFRACTION (2.851 Å)