5HJC

BRD3 second bromodomain in complex with histone H3 acetylation at K18

5HJC の概要

• エントリーDOI: 10.2210/pdb5hjc/pdb
• 関連するPDBエントリー: 5HJB 5HJD
• 分子名称: Bromodomain-containing protein 3, peptide of Histone H3.1, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: complex, brd3, bromodomain, histone peptide, acetyllysine, h3k18, signaling protein-peptide complex, signaling protein/peptide
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : Q15059 P68431
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14185.85

構造登録者

Li, Y.Y.,Zhao, D.,Guan, H.P.,Li, H.T. (登録日: 2016-01-13, 公開日: 2016-04-20, 最終更新日: 2024-11-13)

主引用文献

Li, Y.Y.,Sabari, B.R.,Panchenko, T.,Wen, H.,Zhao, D.,Guan, H.P.,Wan, L.,Huang, H.,Tang, Z.,Zhao, Y.,Roeder, R.G.,Shi, X.,Allis, C.D.,Li, H.T.
Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain
Mol.Cell, 62:181-193, 2016

PubMed Abstract: Recognition of histone covalent modifications by chromatin-binding protein modules ("readers") constitutes a major mechanism for epigenetic regulation, typified by bromodomains that bind acetyllysine. Non-acetyl histone lysine acylations (e.g., crotonylation, butyrylation, propionylation) have been recently identified, but readers that prefer these acylations have not been characterized. Here we report that the AF9 YEATS domain displays selectively higher binding affinity for crotonyllysine over acetyllysine. Structural studies revealed an extended aromatic sandwiching cage with crotonyl specificity arising from π-aromatic and hydrophobic interactions between crotonyl and aromatic rings. These features are conserved among the YEATS, but not the bromodomains. Using a cell-based model, we showed that AF9 co-localizes with crotonylated histone H3 and positively regulates gene expression in a YEATS domain-dependent manner. Our studies define the evolutionarily conserved YEATS domain as a family of crotonyllysine readers and specifically demonstrate that the YEATS domain of AF9 directly links histone crotonylation to active transcription.

PubMed: 27105114
DOI: 10.1016/j.molcel.2016.03.028

実験手法

X-RAY DIFFRACTION (2.6 Å)