5HJ0

Crystal Structure of Mis18 'Yippee-like' Domain

Summary for 5HJ0

• Entry DOI: 10.2210/pdb5hj0/pdb
• Descriptor: Kinetochore protein mis18, ZINC ION (3 entities in total)
• Functional Keywords: centromere, mis18, ligase
• Biological source: Schizosaccharomyces pombe (Fission yeast)
• Cellular location: Cytoplasm : Q9P802
• Total number of polymer chains: 3
• Total formula weight: 41594.42

Authors

Medina-Pritchard, B.,Subramanian, L.,Allshire, R.,Arockia Jeyaprakash, A. (deposition date: 2016-01-12, release date: 2016-03-09, Last modification date: 2024-05-08)

Primary citation

Subramanian, L.,Medina-Pritchard, B.,Barton, R.,Spiller, F.,Kulasegaran-Shylini, R.,Radaviciute, G.,Allshire, R.C.,Arockia Jeyaprakash, A.
Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization.
Embo Rep., 17:496-507, 2016

PubMed Abstract: Mis18 is a key regulator responsible for the centromere localization of the CENP-A chaperone Scm3 in Schizosaccharomyces pombe and HJURP in humans, which establishes CENP-A chromatin that defines centromeres. The molecular and structural determinants of Mis18 centromere targeting remain elusive. Here, by combining structural, biochemical, and yeast genetic studies, we show that the oligomerization of S. pombe Mis18, mediated via its conserved N-terminal Yippee-like domain, is crucial for its centromere localization and function. The crystal structure of the N-terminal Yippee-like domain reveals a fold containing a cradle-shaped pocket that is implicated in protein/nucleic acid binding, which we show is required for Mis18 function. While the N-terminal Yippee-like domain forms a homodimer in vitro and in vivo, full-length Mis18, including the C-terminal α-helical domain, forms a homotetramer in vitro We also show that the Yippee-like domains of human Mis18α/Mis18β interact to form a heterodimer, implying a conserved structural theme for Mis18 regulation.

PubMed: 26921242
DOI: 10.15252/embr.201541520

Experimental method

X-RAY DIFFRACTION (2.64 Å)