Crystal structure of glycine sarcosine N-methyltransferase from Methanohalophilus portucalensis in complex with betaine

Summary for 5HIJ

Related5HII 5HIK 5HIL 5HIM 5GWX 5H02
DescriptorGlycine sarcosine N-methyltransferase, TRIMETHYL GLYCINE (3 entities in total)
Functional Keywordss-adenosylmethionine-dependent methyltransferases (sam or adomet-mtase), class i, monomethylation of glycine and sarcosine, rate-limiting enzyme in betaine biosynthesis, betaine-mediated feedback inhibition, transferase
Biological sourceMethanohalophilus portucalensis FDF-1
Total number of polymer chains1
Total molecular weight32918.44
Lee, Y.R.,Lin, T.S.,Lai, S.J.,Liu, M.S.,Lai, M.C.,Chan, N.L. (deposition date: 2016-01-12, release date: 2016-11-23, Last modification date: 2017-01-18)
Primary citation
Lee, Y.R.,Lin, T.S.,Lai, S.J.,Liu, M.S.,Lai, M.C.,Chan, N.L.
Structural Analysis of Glycine Sarcosine N-methyltransferase from Methanohalophilus portucalensis Reveals Mechanistic Insights into the Regulation of Methyltransferase Activity
Sci Rep, 6:38071-38071, 2016
PubMed: 27934872 (PDB entries with the same primary citation)
DOI: 10.1038/srep38071
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.2101003.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5hij
no rotation
Molmil generated image of 5hij
rotated about x axis by 90°
Molmil generated image of 5hij
rotated about y axis by 90°